解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Helichrysum Mill. (Asteraceae) species have been used in folk medicine for thousands of years in the world. The in vitro cytogenetic effects in human lymphocytes of nine Helichrysum taxa used in Turkey folk medicine were investigated. Blood samples were obtained from healthy donors, non-smoking volunteers, which were ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-009-9193-0
更新日期:2009-01-01 00:00:00
abstract::Vasculogenesis, the formation of blood vessels in embryonic or fetal tissue mediated by immature vascular cells (i.e., angioblasts), is poorly understood. Here we report a summary of our recent studies on the identification of a population of vascular progenitor cells (VPCs) in human fetal aorta. These undifferentiate...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-008-9167-7
更新日期:2008-09-01 00:00:00
abstract::The cancer stem cell hypothesis posits that tumors are derived from a single cancer-initiating cell with stem cell properties. The task of identifying and characterizing cancer-initiating cells with stem cell properties at the single cell level has proven technically difficult because of the scarcity of the cancer ste...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-008-9173-9
更新日期:2008-09-01 00:00:00
abstract::It is well known that the characteristics of cell lines possibly alter when cell lines are at high-passage number because of the environmental selection. We do not know whether non-permissive or low-permissive cell lines could become permissive or more permissive to virus infection after over-high passage. In the pres...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-008-9158-8
更新日期:2008-07-01 00:00:00
abstract::In early development of animals, hedgehog (Hh) genes function as morphogen in the axis determination and the organ formation. In Xenopus, three hedgehog genes, sonic (shh), banded (bhh), and cephalic (chh), were identified and might organize various tissues and organs in embryogenesis. Here, we report the spatial and ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-008-9127-2
更新日期:2008-06-01 00:00:00
abstract::Specific respiration rate ( [Formula: see text]) is a key parameter to understand cell metabolism and physiological state, providing useful information for process supervision and control. In this work, we cultivated different insect cells in a very controlled environment, being able to measure [Formula: see text]. Sp...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-007-9118-8
更新日期:2008-05-01 00:00:00
abstract::Aim We studied the role of mini-TyrRS and mini-TrpRS in angiogenesis by using small interfering RNA-mediated mini-TyrRS/mini-TrpRS knockout in hypoxic culture of human umbilical vein endothelial cells. Methods SiRNA was used as the main method to inhibited the gene function. Silencing efficiency was assayed by real-ti...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-008-9151-2
更新日期:2008-03-01 00:00:00
abstract::We have developed a generic transient transfection process at 100 L scale, using HEK293-EBNA cells and PEI as the transfection reagent for the production of recombinant IgG. The process, including large-scale plasmid preparation, expression at bioreactor scale, capture, purification and, if necessary, endotoxin remova...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-008-9135-2
更新日期:2008-02-01 00:00:00
abstract::Protein delivery represents a powerful tool for experiments in live cells including studies of protein-protein interactions, protein interference with blocking antibodies, intracellular trafficking and protein or peptide biological functions. Most available reagents dedicated to the protein delivery allow efficient cr...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-007-9102-3
更新日期:2008-01-01 00:00:00
abstract::Therapeutic antibody IgG1 has two N-linked oligosaccharide chains bound to the Fc region. The oligosaccharides are of the complex biantennary type, composed of a trimannosyl core structure with the presence or absence of core fucose, bisecting N-acetylglucosamine (GlcNAc), galactose, and terminal sialic acid, which gi...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-007-9103-2
更新日期:2007-12-01 00:00:00
abstract::Fishes have been recently recognized as a suitable model organism to study vertebrate physiological processes, in particular skeletal development and tissue mineralization. However, there is a lack of well characterized in vitro cell systems derived from fish calcified tissues. We describe here a protocol that was suc...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-007-9098-8
更新日期:2007-09-01 00:00:00
abstract::The neurite outgrowth of PC12 cells on collagen-coated glass plates under light emitting diode (LED) irradiation at several wavelengths (i.e., 455, 470, 525, 600, 630, 880 and 945 nm) was investigated. No neurite outgrowth was observed during cultivation under irradiation from the lamp of an inverted light microscope ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-007-9087-y
更新日期:2007-07-01 00:00:00
abstract::Electroporation is a way to induce nanometersized membrane pore for exogenous substances delivery into cytoplasm using an artificial electric field. Now it was widely used for molecules transfer especially in molecular experiments and genetic aspects. In recent years, modern electroporation on the embryo was developed...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-007-9082-3
更新日期:2007-06-01 00:00:00
abstract::The relatively recent discovery of miRNAs has added a completely new dimension to the study of the regulation of gene expression. The mechanism of action of miRNAs, the conservation between diverse species and the fact that each miRNA can regulate a number of targets and phenotypes clearly indicates the importance of ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-007-9049-4
更新日期:2007-04-01 00:00:00
abstract::A new compound, rotenoid isoflavone glycoside named, 6'-O-beta-D: -glucopyranosyl-12a-hydroxydalpanol was isolated from the methanolic (MeOH) fruit extract of Amorpha fruticosa LINNE by means of multi-stage column chromatography. Immuno-modulatory activities of this new glycoside were compared with the partitioned fra...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-006-9040-5
更新日期:2006-11-01 00:00:00
abstract::The UFL-AG-286 cell line, established from embryonic tissue of the lepidopteran insect Anticarsia gemmatalis, has been identified as a good candidate to be used as a cellular substrate in the development of a process for in vitro production of the Anticarsia gemmatalis multicapsid nucleopolyhedrovirus, a baculovirus w...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-006-9042-3
更新日期:2006-10-01 00:00:00
abstract::In this investigation, clastogenic effects of Thymus kotschyanus var. glabrescens Boiss. extract (TE) and anticlastogenic effects of this extract against Mitomycin C (MMC) induced chromosome damage have been evaluated in human peripheral blood lymphocytes in vitro. Two series of experiments were conducted. In the firs...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-006-9015-6
更新日期:2006-06-01 00:00:00
abstract::We describe a novel fully automated high-throughput time-lapse microscopy system and evaluate its performance for precisely tracking the motility of several glioma and osteoblastic cell lines. Use of this system revealed cell motility behavior not discernable with conventional techniques by collecting data (1) from cl...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-006-9006-7
更新日期:2006-05-01 00:00:00
abstract::Early developments in serum-free media led to a variety of formulations in which components normally provided in serum and required for growth (insulin, transferrin, lipid supplements, trace elements) and poorly defined components (extracts, hydrolysates) were added to defined basal media. These additives were mostly ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-006-9002-y
更新日期:2006-03-01 00:00:00
abstract::The development of specific catalytic inhibitors for the serine protease urokinase-type plasminogen activator (uPA) has been hindered due to difficulties in producing sufficient amounts of active recombinant uPA that is catalytically equivalent to native uPA. The purpose of this study was to develop an efficient syste...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-005-4637-7
更新日期:2005-09-01 00:00:00
abstract::We have studied parameters for optimizing the Spodoptera frugiperda (Sf9) cell culture and viral infection for the production of Anticarsia gemmatalis multiple nucleopolyhedrosis virus (AgMNPV) polyhedra inclusion bodies (PIBs) in shaker-Schott or spinner bottles and bioreactors. We have assayed the k(L)a of the syste...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-005-3175-7
更新日期:2005-06-01 00:00:00
abstract::A differentiation method of human bone marrow mesenchymal stem cells (MSCs) to chondrocytes was developed for the construction of a three-dimensional (3D) cartilage tissue. The adhesive cells, which were isolated from a human bone marrow aspirate were embedded in type I collagen in a poly-L: -lactate-glycolic acid cop...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-005-3751-x
更新日期:2005-01-01 00:00:00
abstract::The cAMP and Ca(2+) signaling pathways activate the transcription factor CREB through its phosphorylation at Serine 133. Activation of CREB is involved in the regulation of various biological phenomena. To understand further the mechanisms of the regulation of CREB activity in response to activation of the cAMP and Ca...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-005-3763-6
更新日期:2005-01-01 00:00:00
abstract::In the present manuscript, the methods required to generate purified cultures of mature adipocytes, as well as stromal vascular cells, from the same isolation are detailed. Also, we describe the in vitro conditions for the dedifferentiation of the isolated mature adipocytes. These two types of cells may be used to ree...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-005-2602-0
更新日期:2004-10-01 00:00:00
abstract::Hepatocytes self-assemble in culture to form compacted spherical aggregates, or spheroids, that mimic the structure of the liver by forming tight junctions and bile canalicular channels. Hepatocyte spheroids thus resemble the liver to a great extent. However, liver tissue contains other cell types and has bile ducts a...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-004-7996-6
更新日期:2004-07-01 00:00:00
abstract::A hallmark of human cancer cells is immortal cell growth, which is associated with telomere maintenance by telomerase. The transcriptional regulation of the human telomerase reverse transcriptase (hTERT) gene is a major mechanism that negatively and positively controls telomerase activity in normal and cancer cells, r...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-004-5122-4
更新日期:2004-06-01 00:00:00
abstract::The potential of spent medium to support the growth and recombinant protein production of High-Fivetrade mark cells was investigated. Growth in medium consisting of three parts fresh and one part spent medium was comparable to that in fresh medium (maximal specific growth rates of 0.028 and 0.029 h(-1), and maximal ce...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/B:CYTO.0000043413.53044.fa
更新日期:2004-01-01 00:00:00
abstract::Oral tolerance is the systemic immunological unresponsiveness that occurs after feeding protein antigens. Its physiological role is thought to be the prevention of hypersensitivity to food antigens, and its therapeutic use to treat inflammatory diseases has been suggested. Although it has been shown that CD4(+) T cell...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/b:cyto.0000039918.80472.0e
更新日期:2003-11-01 00:00:00
abstract::Carvacrol, the predominant monoterpene in many essential oils of Labitae including Origanum, Satureja, Thymbra, Thymus, and Corydothymus has substantial antibacterial, antifungal, antihelmintic, insecticial, analgesic and antioxidant activities. Approximately 75% of lung cancer is non-small cell carcinoma (NSCLC) whic...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/b:cyto.0000039917.60348.45
更新日期:2003-11-01 00:00:00
abstract::The Baculovirus Expression Vector System has become widely used for the production of recombinant proteins for research and diagnostics. Serum-free culture media able to support high cell densities have been developed for the large scale culture of insect cells. While serum elimination aims at avoiding the risks assoc...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/B:CYTO.0000009816.65227.84
更新日期:2003-07-01 00:00:00
abstract::The capacity of hepatocytes and cholangiocytes to contribute to their own maintenance has long been recognized. More recently, studies have indicated the presence of both intra-hepatic and extra-hepatic stem/progenitor cell populations. The intraorgan compartment probably derives primarily from the biliary tree, most ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1024826823194
更新日期:2003-03-01 00:00:00
abstract::To characterize the effects of cetyltriethylammonium bromide (CTAB) on the transcription of gp64 promoter from Bombyx mori nucleopolyhedrovirus (BmNPV), the plasmid pBmgp64Luc used in transient expression assay system was constructed by using the luciferase gene as a reporter under the control of BmNPV gp64 promoter. ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1024231023015
更新日期:2003-01-01 00:00:00
abstract::In this article, we describe a new method that facilitates to isolate mammalian cells inducible hyper-producing heterologous proteins. This method uses the tetracycline-inducible system to express both the selection marker and the heterologous gene, therefore, allows to increase the selection pressure by reducing the ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1023945517446
更新日期:2002-11-01 00:00:00
abstract::We screened for biological activity which induces neurite outgrowth in vitro from 300 species of marine algae from along the Japan coast for possible use as a treatment for the lack of neurotrophic factor which is considered to be a cause of Alzheimer's disease. In this study, we evaluated the neurite outgrowth promot...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1023974323807
更新日期:2002-11-01 00:00:00
abstract::A sequential multiprobe fluorescence in situ hybridization technique was developed to study the 13, 18, 21, X and Y chromatid segregation in human lymphocytes anaphases cultures without antimitotic treatment. This method was used to know if exist any different chromosomes segregation in lymphocytes from Down syndrome ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1023945218123
更新日期:2002-09-01 00:00:00
abstract::The subcellular localisation of doxorubicin and Victoria Blue BO (VBBO) in a murine mammary tumour cell line EMT6-S, and the resistant sub-lineEMT6-R was studied, using confocal microscopy, in order to investigate their sites of action. In cells treated with doxorubicin (10 mu M) for 90 min, the pattern of intracellul...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1022435829894
更新日期:2002-05-01 00:00:00
abstract::Chemical genomics is a new research paradigm with importantapplications in drug discovery. It links genomic targets withsmall-molecule chemistries thereby allowing for efficient targetvalidation and lead compound identification. ACADIA'schemical-genomics platform consists of a large and diverse small-moleculelibrary (...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1021169023731
更新日期:2002-01-01 00:00:00
abstract::There is a constant and growing interest in exploitingadenoviruses as vectors for gene therapy when transientexpression of a therapeutic protein is necessary. Therequirement for an increased viral titre has prompted asearch for techniques by which this virus may be assayedwith greater speed and simplicity. Conventiona...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1021106116887
更新日期:2002-01-01 00:00:00
abstract::Menadione-catalyzed H(2)O(2) production by viable animal cells was proportional to the viable cell number, and H(2)O(2) production decreased with increasing cytotoxic effects after the incubation of cells with cytotoxic compounds. The cytotoxic effects of food additives, pesticides, antibiotics, heavy metals, phytotox...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1020580818979
更新日期:2001-11-01 00:00:00
abstract::Explant cultures of porcine coronary artery provided a coculture model, used as a paradigm of arterial wall in contact with vascular prosthesis which allowed the study of spatial and temporal changes in cell phenotype. First cells emerging from the explant had an endothelial phenotype monitored by cytoimmunostaining. ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1016195029359
更新日期:2001-09-01 00:00:00